The peptide AC 2 isolated from Bacillus-treated Trapa japonica fruit extract rescues DHT (dihydrotestosterone)-treated human dermal papilla cells and mediates mTORC1 signaling for autophagy and apoptosis suppression.
Identifieur interne : 000193 ( Main/Exploration ); précédent : 000192; suivant : 000194The peptide AC 2 isolated from Bacillus-treated Trapa japonica fruit extract rescues DHT (dihydrotestosterone)-treated human dermal papilla cells and mediates mTORC1 signaling for autophagy and apoptosis suppression.
Auteurs : Gun He Nam [Corée du Sud] ; Kyung-Jo Jo [Corée du Sud] ; Ye-Seul Park [Corée du Sud] ; Hye Won Kawk [Corée du Sud] ; Je-Geun Yoo [Corée du Sud] ; Jin Dong Jang [Corée du Sud] ; Sang Moon Kang [Corée du Sud] ; Sang-Yong Kim [Corée du Sud] ; Young-Min Kim [Corée du Sud]Source :
- Scientific reports [ 2045-2322 ] ; 2019.
Descripteurs français
- KwdFr :
- 5alpha-Dihydrotestostérone (pharmacologie), Alopécie (anatomopathologie), Alopécie (métabolisme), Alopécie (prévention et contrôle), Apoptose (effets des médicaments et des substances chimiques), Autophagie (effets des médicaments et des substances chimiques), Bacillus (physiologie), Cellules cultivées (MeSH), Complexe-1 cible mécanistique de la rapamycine (métabolisme), Cuir chevelu (cytologie), Cuir chevelu (effets des médicaments et des substances chimiques), Cytoprotection (effets des médicaments et des substances chimiques), Derme (cytologie), Derme (effets des médicaments et des substances chimiques), Derme (métabolisme), Extraits de plantes (composition chimique), Extraits de plantes (pharmacologie), Follicule pileux (cytologie), Follicule pileux (effets des médicaments et des substances chimiques), Follicule pileux (métabolisme), Fragments peptidiques (composition chimique), Fragments peptidiques (pharmacologie), Fruit (composition chimique), Humains (MeSH), Lythraceae (composition chimique), Lythraceae (microbiologie), Transduction du signal (effets des médicaments et des substances chimiques).
- MESH :
- anatomopathologie : Alopécie.
- composition chimique : Extraits de plantes, Fragments peptidiques, Fruit, Lythraceae.
- cytologie : Cuir chevelu, Derme, Follicule pileux.
- effets des médicaments et des substances chimiques : Apoptose, Autophagie, Cuir chevelu, Cytoprotection, Derme, Follicule pileux, Transduction du signal.
- microbiologie : Lythraceae.
- métabolisme : Alopécie, Complexe-1 cible mécanistique de la rapamycine, Derme, Follicule pileux.
- pharmacologie : 5alpha-Dihydrotestostérone, Extraits de plantes, Fragments peptidiques.
- physiologie : Bacillus.
- prévention et contrôle : Alopécie.
- Cellules cultivées, Humains.
English descriptors
- KwdEn :
- Alopecia (metabolism), Alopecia (pathology), Alopecia (prevention & control), Apoptosis (drug effects), Autophagy (drug effects), Bacillus (physiology), Cells, Cultured (MeSH), Cytoprotection (drug effects), Dermis (cytology), Dermis (drug effects), Dermis (metabolism), Dihydrotestosterone (pharmacology), Fruit (chemistry), Hair Follicle (cytology), Hair Follicle (drug effects), Hair Follicle (metabolism), Humans (MeSH), Lythraceae (chemistry), Lythraceae (microbiology), Mechanistic Target of Rapamycin Complex 1 (metabolism), Peptide Fragments (chemistry), Peptide Fragments (pharmacology), Plant Extracts (chemistry), Plant Extracts (pharmacology), Scalp (cytology), Scalp (drug effects), Signal Transduction (drug effects).
- MESH :
- chemical , chemistry : Peptide Fragments, Plant Extracts.
- chemical , metabolism : Mechanistic Target of Rapamycin Complex 1.
- chemical , pharmacology : Dihydrotestosterone, Peptide Fragments, Plant Extracts.
- chemistry : Fruit, Lythraceae.
- cytology : Dermis, Hair Follicle, Scalp.
- drug effects : Apoptosis, Autophagy, Cytoprotection, Dermis, Hair Follicle, Scalp, Signal Transduction.
- metabolism : Alopecia, Dermis, Hair Follicle.
- microbiology : Lythraceae.
- pathology : Alopecia.
- physiology : Bacillus.
- prevention & control : Alopecia.
- Cells, Cultured, Humans.
Abstract
The Trapa japonica fruit is a natural plant growing in ponds with its roots in the mud. It has long been used as a home remedy for many diseases; however, a major problem with this kind of natural extract is the multicomponents-multitargets for diseases. Such problems make it difficult to identify the mechanism of action. Another problem is quality control and consistency. The aim of this research was to isolate a single bioactive compound (peptide) derived from the Trapa japonica fruit. The research was conducted with various experimental techniques, such as fermentation and liquid chromatography, to isolate a peptide. We isolated the AC 2 peptide from Trapa japonica fruit and found it to be promising on human dermal papilla cells. Dihydrotestosterone (DHT) stresses human dermal papilla cells and is a major cause of hair loss resulting from hormones and environmental factors. The purpose of this research was to develop an understanding of the mechanism by which the AC 2 peptide rescues dihydrotestosterone (DHT)-treated human dermal papilla cells. We explored the effects of the AC 2 peptide on the cell biological functions of human dermal papilla cells (HDPs). HDPs were treated with the AC 2 peptide and DHT. Then, a cytotoxicity assay, flow cytometry, Western blot, immunoprecipitation, and 3D cell culture for immunohistochemistry were conducted to investigate the mTORC1 pathway and suppression of autophagy and apoptosis. In addition, we also synthesized the AC2 peptide as an alternative to the expensive and difficult isolation and purification procedures and confirmed its potential in biomedical applications. We also validated the effects of the synthetic AC2 peptide as well as the isolated and purified AC2 peptide and established their similarity. Although extensive research has been carried out on natural extracts, few single studies have isolated and separated a bioactive peptide (single compound).
DOI: 10.1038/s41598-019-53347-3
PubMed: 31729428
PubMed Central: PMC6858360
Affiliations:
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xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054</wicri:regionArea><wicri:noRegion>34054</wicri:noRegion></affiliation></author><author><name sortKey="Kawk, Hye Won" sort="Kawk, Hye Won" uniqKey="Kawk H" first="Hye Won" last="Kawk">Hye Won Kawk</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054</wicri:regionArea><wicri:noRegion>34054</wicri:noRegion></affiliation></author><author><name sortKey="Yoo, Je Geun" 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University, Daehakro Danwon-gu, Ansan-city, Gyenggi-do, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Food Science & Bio Technology, Shinansan University, Daehakro Danwon-gu, Ansan-city, Gyenggi-do</wicri:regionArea><wicri:noRegion>Gyenggi-do</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Young Min" sort="Kim, Young Min" uniqKey="Kim Y" first="Young-Min" last="Kim">Young-Min Kim</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054, South Korea. ym1938@naver.com.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 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sortKey="Nam, Gun He" sort="Nam, Gun He" uniqKey="Nam G" first="Gun He" last="Nam">Gun He Nam</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054</wicri:regionArea><wicri:noRegion>34054</wicri:noRegion></affiliation></author><author><name sortKey="Jo, Kyung Jo" sort="Jo, Kyung Jo" uniqKey="Jo K" first="Kyung-Jo" last="Jo">Kyung-Jo Jo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054</wicri:regionArea><wicri:noRegion>34054</wicri:noRegion></affiliation></author><author><name sortKey="Park, Ye Seul" sort="Park, Ye Seul" uniqKey="Park Y" first="Ye-Seul" last="Park">Ye-Seul Park</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054</wicri:regionArea><wicri:noRegion>34054</wicri:noRegion></affiliation></author><author><name sortKey="Kawk, Hye Won" sort="Kawk, Hye Won" uniqKey="Kawk H" first="Hye Won" last="Kawk">Hye Won Kawk</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054</wicri:regionArea><wicri:noRegion>34054</wicri:noRegion></affiliation></author><author><name sortKey="Yoo, Je Geun" sort="Yoo, Je Geun" uniqKey="Yoo J" first="Je-Geun" last="Yoo">Je-Geun Yoo</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054</wicri:regionArea><wicri:noRegion>34054</wicri:noRegion></affiliation></author><author><name sortKey="Jang, Jin Dong" sort="Jang, Jin Dong" uniqKey="Jang J" first="Jin Dong" last="Jang">Jin Dong Jang</name><affiliation wicri:level="3"><nlm:affiliation>Doori Cosmetics Co.,Ltd., 11F Galaxy Tower, 175, Saimdang-ro, Seocho-gu, Seoul, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Doori Cosmetics Co.,Ltd., 11F Galaxy Tower, 175, Saimdang-ro, Seocho-gu, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kang, Sang Moon" sort="Kang, Sang Moon" uniqKey="Kang S" first="Sang Moon" last="Kang">Sang Moon Kang</name><affiliation wicri:level="1"><nlm:affiliation>ANPEP INC. 13, Oksansandan 1-ro, Oksan-myeon, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>ANPEP INC. 13, Oksansandan 1-ro, Oksan-myeon, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do</wicri:regionArea><wicri:noRegion>Chungcheongbuk-do</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Sang Yong" sort="Kim, Sang Yong" uniqKey="Kim S" first="Sang-Yong" last="Kim">Sang-Yong Kim</name><affiliation wicri:level="1"><nlm:affiliation>Department of Food Science & Bio Technology, Shinansan University, Daehakro Danwon-gu, Ansan-city, Gyenggi-do, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Food Science & Bio Technology, Shinansan University, Daehakro Danwon-gu, Ansan-city, Gyenggi-do</wicri:regionArea><wicri:noRegion>Gyenggi-do</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Young Min" sort="Kim, Young Min" uniqKey="Kim Y" first="Young-Min" last="Kim">Young-Min Kim</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054, South Korea. ym1938@naver.com.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054</wicri:regionArea><wicri:noRegion>34054</wicri:noRegion></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alopecia (metabolism)</term><term>Alopecia (pathology)</term><term>Alopecia (prevention & control)</term><term>Apoptosis (drug effects)</term><term>Autophagy (drug effects)</term><term>Bacillus (physiology)</term><term>Cells, Cultured (MeSH)</term><term>Cytoprotection (drug effects)</term><term>Dermis (cytology)</term><term>Dermis (drug effects)</term><term>Dermis (metabolism)</term><term>Dihydrotestosterone (pharmacology)</term><term>Fruit (chemistry)</term><term>Hair Follicle (cytology)</term><term>Hair Follicle (drug effects)</term><term>Hair Follicle (metabolism)</term><term>Humans (MeSH)</term><term>Lythraceae (chemistry)</term><term>Lythraceae (microbiology)</term><term>Mechanistic Target of Rapamycin Complex 1 (metabolism)</term><term>Peptide Fragments (chemistry)</term><term>Peptide Fragments (pharmacology)</term><term>Plant Extracts (chemistry)</term><term>Plant Extracts (pharmacology)</term><term>Scalp (cytology)</term><term>Scalp (drug effects)</term><term>Signal Transduction (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>5alpha-Dihydrotestostérone (pharmacologie)</term><term>Alopécie (anatomopathologie)</term><term>Alopécie (métabolisme)</term><term>Alopécie (prévention et contrôle)</term><term>Apoptose (effets des médicaments et des substances chimiques)</term><term>Autophagie (effets des médicaments et des substances chimiques)</term><term>Bacillus (physiologie)</term><term>Cellules cultivées (MeSH)</term><term>Complexe-1 cible mécanistique de la rapamycine (métabolisme)</term><term>Cuir chevelu (cytologie)</term><term>Cuir chevelu (effets des médicaments et des substances chimiques)</term><term>Cytoprotection (effets des médicaments et des substances chimiques)</term><term>Derme (cytologie)</term><term>Derme (effets des médicaments et des substances chimiques)</term><term>Derme (métabolisme)</term><term>Extraits de plantes (composition chimique)</term><term>Extraits de plantes (pharmacologie)</term><term>Follicule pileux (cytologie)</term><term>Follicule pileux (effets des médicaments et des substances chimiques)</term><term>Follicule pileux (métabolisme)</term><term>Fragments peptidiques (composition chimique)</term><term>Fragments peptidiques (pharmacologie)</term><term>Fruit (composition chimique)</term><term>Humains (MeSH)</term><term>Lythraceae (composition chimique)</term><term>Lythraceae (microbiologie)</term><term>Transduction du signal (effets des médicaments et des substances chimiques)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Peptide Fragments</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Mechanistic Target of Rapamycin Complex 1</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Dihydrotestosterone</term><term>Peptide Fragments</term><term>Plant Extracts</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Alopécie</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Fruit</term><term>Lythraceae</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Extraits de plantes</term><term>Fragments peptidiques</term><term>Fruit</term><term>Lythraceae</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Cuir chevelu</term><term>Derme</term><term>Follicule pileux</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Dermis</term><term>Hair Follicle</term><term>Scalp</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Apoptosis</term><term>Autophagy</term><term>Cytoprotection</term><term>Dermis</term><term>Hair Follicle</term><term>Scalp</term><term>Signal Transduction</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Apoptose</term><term>Autophagie</term><term>Cuir chevelu</term><term>Cytoprotection</term><term>Derme</term><term>Follicule pileux</term><term>Transduction du signal</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Alopecia</term><term>Dermis</term><term>Hair Follicle</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Lythraceae</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lythraceae</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Alopécie</term><term>Complexe-1 cible mécanistique de la rapamycine</term><term>Derme</term><term>Follicule pileux</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Alopecia</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>5alpha-Dihydrotestostérone</term><term>Extraits de plantes</term><term>Fragments peptidiques</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Bacillus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Bacillus</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Alopecia</term></keywords><keywords scheme="MESH" qualifier="prévention et contrôle" xml:lang="fr"><term>Alopécie</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cells, Cultured</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules cultivées</term><term>Humains</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Trapa japonica fruit is a natural plant growing in ponds with its roots in the mud. It has long been used as a home remedy for many diseases; however, a major problem with this kind of natural extract is the multicomponents-multitargets for diseases. Such problems make it difficult to identify the mechanism of action. Another problem is quality control and consistency. The aim of this research was to isolate a single bioactive compound (peptide) derived from the Trapa japonica fruit. The research was conducted with various experimental techniques, such as fermentation and liquid chromatography, to isolate a peptide. We isolated the AC 2 peptide from Trapa japonica fruit and found it to be promising on human dermal papilla cells. Dihydrotestosterone (DHT) stresses human dermal papilla cells and is a major cause of hair loss resulting from hormones and environmental factors. The purpose of this research was to develop an understanding of the mechanism by which the AC 2 peptide rescues dihydrotestosterone (DHT)-treated human dermal papilla cells. We explored the effects of the AC 2 peptide on the cell biological functions of human dermal papilla cells (HDPs). HDPs were treated with the AC 2 peptide and DHT. Then, a cytotoxicity assay, flow cytometry, Western blot, immunoprecipitation, and 3D cell culture for immunohistochemistry were conducted to investigate the mTORC1 pathway and suppression of autophagy and apoptosis. In addition, we also synthesized the AC2 peptide as an alternative to the expensive and difficult isolation and purification procedures and confirmed its potential in biomedical applications. We also validated the effects of the synthetic AC2 peptide as well as the isolated and purified AC2 peptide and established their similarity. Although extensive research has been carried out on natural extracts, few single studies have isolated and separated a bioactive peptide (single compound).</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31729428</PMID><DateCompleted><Year>2020</Year><Month>11</Month><Day>12</Day></DateCompleted><DateRevised><Year>2020</Year><Month>11</Month><Day>14</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>11</Month><Day>15</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>The peptide AC 2 isolated from Bacillus-treated Trapa japonica fruit extract rescues DHT (dihydrotestosterone)-treated human dermal papilla cells and mediates mTORC1 signaling for autophagy and apoptosis suppression.</ArticleTitle><Pagination><MedlinePgn>16903</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41598-019-53347-3</ELocationID><Abstract><AbstractText>The Trapa japonica fruit is a natural plant growing in ponds with its roots in the mud. It has long been used as a home remedy for many diseases; however, a major problem with this kind of natural extract is the multicomponents-multitargets for diseases. Such problems make it difficult to identify the mechanism of action. Another problem is quality control and consistency. The aim of this research was to isolate a single bioactive compound (peptide) derived from the Trapa japonica fruit. The research was conducted with various experimental techniques, such as fermentation and liquid chromatography, to isolate a peptide. We isolated the AC 2 peptide from Trapa japonica fruit and found it to be promising on human dermal papilla cells. Dihydrotestosterone (DHT) stresses human dermal papilla cells and is a major cause of hair loss resulting from hormones and environmental factors. The purpose of this research was to develop an understanding of the mechanism by which the AC 2 peptide rescues dihydrotestosterone (DHT)-treated human dermal papilla cells. We explored the effects of the AC 2 peptide on the cell biological functions of human dermal papilla cells (HDPs). HDPs were treated with the AC 2 peptide and DHT. Then, a cytotoxicity assay, flow cytometry, Western blot, immunoprecipitation, and 3D cell culture for immunohistochemistry were conducted to investigate the mTORC1 pathway and suppression of autophagy and apoptosis. In addition, we also synthesized the AC2 peptide as an alternative to the expensive and difficult isolation and purification procedures and confirmed its potential in biomedical applications. We also validated the effects of the synthetic AC2 peptide as well as the isolated and purified AC2 peptide and established their similarity. Although extensive research has been carried out on natural extracts, few single studies have isolated and separated a bioactive peptide (single compound).</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nam</LastName><ForeName>Gun He</ForeName><Initials>GH</Initials><AffiliationInfo><Affiliation>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jo</LastName><ForeName>Kyung-Jo</ForeName><Initials>KJ</Initials><AffiliationInfo><Affiliation>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Ye-Seul</ForeName><Initials>YS</Initials><AffiliationInfo><Affiliation>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kawk</LastName><ForeName>Hye Won</ForeName><Initials>HW</Initials><AffiliationInfo><Affiliation>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yoo</LastName><ForeName>Je-Geun</ForeName><Initials>JG</Initials><AffiliationInfo><Affiliation>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jang</LastName><ForeName>Jin Dong</ForeName><Initials>JD</Initials><AffiliationInfo><Affiliation>Doori Cosmetics Co.,Ltd., 11F Galaxy Tower, 175, Saimdang-ro, Seocho-gu, Seoul, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kang</LastName><ForeName>Sang Moon</ForeName><Initials>SM</Initials><AffiliationInfo><Affiliation>ANPEP INC. 13, Oksansandan 1-ro, Oksan-myeon, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Sang-Yong</ForeName><Initials>SY</Initials><AffiliationInfo><Affiliation>Department of Food Science & Bio Technology, Shinansan University, Daehakro Danwon-gu, Ansan-city, Gyenggi-do, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Young-Min</ForeName><Initials>YM</Initials><AffiliationInfo><Affiliation>Department of Biological science and Biotechnology, College of Life science and Nano technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054, South Korea. ym1938@naver.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>11</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010446">Peptide Fragments</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010936">Plant Extracts</NameOfSubstance></Chemical><Chemical><RegistryNumber>08J2K08A3Y</RegistryNumber><NameOfSubstance UI="D013196">Dihydrotestosterone</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.11.1</RegistryNumber><NameOfSubstance UI="D000076222">Mechanistic Target of Rapamycin Complex 1</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="ErratumIn"><RefSource>Sci Rep. 2020 May 19;10(1):8571</RefSource><PMID Version="1">32424185</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000505" MajorTopicYN="N">Alopecia</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName><QualifierName UI="Q000517" MajorTopicYN="N">prevention & control</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017209" MajorTopicYN="N">Apoptosis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001343" MajorTopicYN="N">Autophagy</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001407" MajorTopicYN="N">Bacillus</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019610" MajorTopicYN="N">Cytoprotection</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020405" MajorTopicYN="N">Dermis</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013196" MajorTopicYN="N">Dihydrotestosterone</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005638" MajorTopicYN="N">Fruit</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018859" MajorTopicYN="N">Hair Follicle</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D029561" MajorTopicYN="N">Lythraceae</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000076222" MajorTopicYN="N">Mechanistic Target of Rapamycin Complex 1</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010446" MajorTopicYN="N">Peptide Fragments</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010936" MajorTopicYN="N">Plant Extracts</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012535" MajorTopicYN="N">Scalp</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>07</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>10</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>11</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>11</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31729428</ArticleId><ArticleId IdType="doi">10.1038/s41598-019-53347-3</ArticleId><ArticleId IdType="pii">10.1038/s41598-019-53347-3</ArticleId><ArticleId IdType="pmc">PMC6858360</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Nam, Gun He" sort="Nam, Gun He" uniqKey="Nam G" first="Gun He" last="Nam">Gun He Nam</name></noRegion><name sortKey="Jang, Jin Dong" sort="Jang, Jin Dong" uniqKey="Jang J" first="Jin Dong" last="Jang">Jin Dong Jang</name><name sortKey="Jo, Kyung Jo" sort="Jo, Kyung Jo" uniqKey="Jo K" first="Kyung-Jo" last="Jo">Kyung-Jo Jo</name><name sortKey="Kang, Sang Moon" sort="Kang, Sang Moon" uniqKey="Kang S" first="Sang Moon" last="Kang">Sang Moon Kang</name><name sortKey="Kawk, Hye Won" sort="Kawk, Hye Won" uniqKey="Kawk H" first="Hye Won" last="Kawk">Hye Won Kawk</name><name sortKey="Kim, Sang Yong" sort="Kim, Sang Yong" uniqKey="Kim S" first="Sang-Yong" last="Kim">Sang-Yong Kim</name><name sortKey="Kim, Young Min" sort="Kim, Young Min" uniqKey="Kim Y" first="Young-Min" last="Kim">Young-Min Kim</name><name sortKey="Park, Ye Seul" sort="Park, Ye Seul" uniqKey="Park Y" first="Ye-Seul" last="Park">Ye-Seul Park</name><name sortKey="Yoo, Je Geun" sort="Yoo, Je Geun" uniqKey="Yoo J" first="Je-Geun" last="Yoo">Je-Geun Yoo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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